商品化培养液对人类胚胎发育及妊娠结局的影响

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商品化培养液对人类胚胎发育及妊娠结局的影响

  商品化培养液对人类胚胎发育及妊娠结局的影响

商品化培养液对人类胚胎发育及妊娠结局的影响

  [摘要] 目的 比较两种商品化序贯培养液Cook和Vitrolife对人类胚胎发育潜能及妊娠结局的影响。 方法 回顾性分析2014年8月~2015年5月于南京大学医学院附属鼓楼医院生殖医学中心行体外受精(IVF)或卵胞浆内单精子注射(ICSI)的398例患者的临床资料。根据受精方式与胚胎培养液不同进行分组,IVF周期分为Cook组(n=103例)和Vitrolife组(n=196例),ICSI周期分为Cook组(n=40例)和Vitrolife组(n=59例)。比较同一受精方式下两种培养液组的受精率、正常受精率、卵裂率、可利用胚胎率、囊胚形成率、可利用囊胚率、临床妊娠率、胚胎种植率和早期流产率。 代孕遗产官司 结果 IVF和ICSI周期中,Cook组受精后第3天可利用胚胎率均显著低于Vitrolife组(31.22%比40.61%,33.43%比42.34%,P < 0.05),而可利用囊胚率均显著高于Vitrolife组(55.42%比37.82%,46.39%比30.58%,P < 0.05)。受精后第3天胚胎发育速度方面,Cook组5~7个细胞的胚胎所占比例显著高于Vitrolife组(34.32%比24.23%,P < 0.05),而Vitrolife组8~10个细胞的胚胎所占比例显著高于Cook组(52.22%比42.07%,P < 0.05)。 结论 Vitrolife培养液和Cook培养液对妊娠结局并无显著影响,但Cook培养液可能更有利于囊胚阶段的发育。

商品化培养液对人类胚胎发育及妊娠结局的影响

  [关键词] 胚胎;培养液;培养发育;妊娠结局

  [中图分类号] R321 [文献标识码] A [文章编号] 1674-4721(2016)09(b)-0072-04

  [Abstract] Objective To compare the influence of two commercially sequential media Cook and Vitrolife on development potential of human embryos and the clinical pregnancy outcome. Methods Clinical data of 398 patients conceived through IVF or ICSI from August 2014 to May 2015 in the Reproductive Medicine Center of 备孕社保代缴 the Affiliated Drum Tower Hospital of Nanjing University Medical School were retrospectively analyzed, and according to fertilization methods and embryo culture media they were divided into Cook group (103 cases) and Vitrolife group (196 cases) in IVF; while Cook group (40 cases) and Vitrolife group (59 cases) in ICSI. In the same fertilization mode, the rate of fertilization, normal fertilization, cleavage, avaiable embryo, blastocyst formation, avaiable blastocyst, clinical pregnancy, embryo implantation and early abortion in the Vitrolife group and Cook group were compared. Results In IVF and ICSI cycles, the available embryo rate of the 3 days after fertilization in Cook group was lower than that in the Vitrolife group (31.22% vs 40.61%, 33.43% vs 42.34%, P   [Key words] Embryo; Culture media; Embryonic development; Pregnancy 受孕失败闺蜜代孕新闻 outcome

  人类胚胎培养液经历了30多年的发展,如今市场上已出现多种有效的商品化培养液用于胚胎培养。不同品牌的培养液有不同类型及不同浓度的

商品化培养液对人类胚胎发育及妊娠结局的影响